Abstract: This study was done with the aim of making and testing multimedia program in Biology and Hygiene course by using scientific exploration model in Birjand. The method which is used is Quasi-experimental and the research population consists of the students of a guidance school in Birjand. This study consists of 40 individuals that have been chosen by available method and the students were considered homogeneously with average, parents, educational levels and social status. Educational attainment in high and low levels of cognitive domain was measured in pre-test and post- test. The results of analyzing research by using combined ANOVA and repeated measurement indicates that the educational progress in both levels (upper and lower) of the cognitive area in the experimental group with compare to the control group was significantly higher. On the other hand t-test analysis results showed that the teaching by multimedia method has been increased the period of memories in the students.